Clinical-scale in vitro expansion preserves biological characteristics of cardiac atrial appendage stem cells.

Mesenchymal Stem Cells: History, Isolation and Biology

Mesenchymal stem cells (MSCs) as a kind of adult stem cells possess two properties of long term selfrenewal ability and multilineage differentiation potential into skeletal cell lineages. MSCs were first isolated and described from bone marrow samples. Further investigations have identified several other tissues as alternative sources for these cells. In spite of the clinical importance of MSCs...

Large-Scale Expansion of Human Embryonic and Induced Pluripotent Stem Cells for Cell Therapy Applications

Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

Human Mesenchymal Stem Cells and Their, Clinical Aapplication

There are two main categories for stem cells a cording to their origin: Embryonic Stem Cells and Adult Stem Cell. Mesenchymal stem cell, supporting hematopoetic stem cells in bone marrow, can regenerate tissues such as bone, cartilage, muscle, tendon and fatty tissue. These cells were recognized for the first time by Friedenstein and Petrokova who could isolate theme from rat bone marrow.Mesenc...

Human Cardiac Stem Cells Isolated from Atrial Appendages Stably Express c-kit

The in vivo studies of myocardial infarct using c-ki⁺/Lin⁻ cardiac stem cells (CSCs) are still in the early stage with margin or no beneficial effects for cardiac function. One of the potential reasons may be related to the absence of fully understanding the properties of these cells both in vitro and in vivo. In the present study, we aimed to systematically examine how CSCs adapted to in vitro...